Algal Glyceraldehyde-3-Phosphate Dehydrogenase. Pyridine-Nucleotide Requirements of Two Enzymes Purified from Scenedesmus obliquus
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of hamster glyceraldehyde-3-phosphate dehydrogenase mRNA.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used as an invariant control for gene expression analysis. Only three vertebrate GAPDH cDNAs have been hitherto isolated: human (1), rat (2) and chick (3). In order to get a probe for RNAse-mapping experiments on hamster {Cricetulus griseus) mRNAs, we constructed a cDNA library in pBluescript KSH (St...
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The catalytic interaction of glyceraldehyde-3-phosphate dehydrogenase with glyceraldehyde 3-phosphate has been examined by transient-state kinetic methods. The results confirm previous reports that the apparent Km for oxidative phosphorylation of glyceraldehyde 3-phosphate decreases at least 50-fold when the substrate is generated in a coupled reaction system through the action of aldolase on f...
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Nitrate and phosphate are abundant in urban wastewater. We evaluated the efficiency of nitrate and phosphate removal from wastewater by Scenedesmus obliquus and its use as a medium for Scenedesmus obliquus. Samples were collected from the effluent of central wastewater treatment in Gorgan. This experiment was done by 3 treatments and 6 replications that effluent with 100%, 50% and 0% were dilut...
متن کاملThe nucleotide sequence of 5S rRNA from Scenedesmus obliquus.
The nucleotide sequence of the cytoplasmic 5S ribosomal RNA from Scenedesmusobliquus has been determined using post-labelling techniques. The sequence is closely related to the 5S RNA sequence of Chlorella and contains all the areas of invariant sequence which appear to be conserved in plant cytoplasmic 5S RNA species.
متن کاملReversible Dissociation of Glycolytic Enzymes: Kinetic Studies on Lactate Dehydrogenase, Glyceraldehyde 3-Phosphate Dehydrogenase and Aldolase
e.g. phosphoglucomutase, glucose 6-phosphate dehydrogenase and fructose 6-phosphate kinase. However, if the reaction occurs distant from the anomeric carbon atom of the substrate the enzymes show no anomeric specificity, e.g. hexokinase, glucokinase and glucose 6-phosphatase (Wurster & Hess, 1974~). A search for enzymes catalysing anomerization reactions leads to the following conclusion. For D...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10218.x